Journal: Molecular Cancer

Article Title: Survivin 2α: a novel Survivin splice variant expressed in human malignancies

doi: 10.1186/1476-4598-4-11

Figure Lengend Snippet: Survivin 2α expression (relative to normal tissue).

Article Snippet: Seven fresh frozen primary medulloblastoma tumor samples were obtained from the Cooperative Human Tumor Network (CHTN), after approval through the Columbus Children's Hospital IRB.

Techniques: Expressing

Journal: Molecular Cancer

Article Title: Survivin 2α: a novel Survivin splice variant expressed in human malignancies

doi: 10.1186/1476-4598-4-11

Figure Lengend Snippet: Expression of survivin splice variants in medulloblastoma (relative to survivin)

Article Snippet: Seven fresh frozen primary medulloblastoma tumor samples were obtained from the Cooperative Human Tumor Network (CHTN), after approval through the Columbus Children's Hospital IRB.

Techniques: Expressing

Journal: American Journal of Cancer Research

Article Title: Upregulation of CYP1B1 by hypoxia is mediated by ERα activation in breast cancer cells

doi:

Figure Lengend Snippet: Analysis of HIF-1α and CYP1B1 expression in tissue microarray (TMA) samples from breast cancer patients. (A) Schematic diagram of the tumor stages of each breast tissue core. (B) Hematoxylin and eosin (H&E) staining images provided by the manufacturer. Immunofluorescence images of tumor microarrays (TMA) stained with anti-hypoxia-inducible factor-1 alpha (HIF-1α) (C) and anti-cytochrome P450 1B1 (CYP1B1) (D) antibodies. Images were acquired at 20X magnification using a Leica Thunder microscope. Whole tissue slide images were mosaic merged via LAS-X software. (E) Representative images of immunofluorescence analysis of HIF-1α (green) and CYP1B1 (green) in two slides of ER-negative and positive breast cancer TMA. Immunofluorescence images were acquired using a Leica Thunder microscope. (F) Scatter plots showing fluorescence intensity correlation analysis of HIF-1α and CYP1B1 in 64 ER-positive TMAs. (G) Violin plots of fluorescence intensities of HIF-1α and CYP1B1. Samples were classified into low (immunohistochemical [IHC] score: 1+, 2+) and high (IHC score: 3+) groups according to the estrogen receptor (ER) expression status of the TMA specification sheet, and the correlation between HIF-1α and CYP1B1 expression was analyzed. (H) Analysis of fluorescence intensity of CYP1B1 against HIF1α expression in ER-positive TMA. The fluorescence intensity of CYP1B1 was analyzed by classifying the fluorescence intensity of HIF1-α based on the top and bottom 25% ER expression. *, P<0.0001 versus ER-positive tumor tissue with low HIF1-α fluorescence intensity by Mann-Whitney test.

Article Snippet: Immunofluorescence analysis of tumor microarrays (TMAs) A human breast cancer tissue microarray (TMA, #BC081120e) containing 110 cases was purchased from Biomax (Rockville, MD).

Techniques: Expressing, Microarray, Staining, Immunofluorescence, Microscopy, Software, Fluorescence, Immunohistochemical staining, MANN-WHITNEY

Journal: Molecular Cell

Article Title: Phosphorylation of PDHA by AMPK Drives TCA Cycle to Promote Cancer Metastasis

doi: 10.1016/j.molcel.2020.09.018

Figure Lengend Snippet: AMPK-PDHc Axis Is Activated in Advanced Breast Cancer Patients, and Its Activation Predicts Poor Metastasis-free Survival (A) PDHA S293 phosphorylation was determined in AMPKα1 knockdown cells. (B) PDHA S293 phosphorylation was determined in control and AMPKα1 knockdown cells with or without A-769662 (100 μM) treatment. (C) The interaction between PDHA and PDHK1 was determined in control and AMPKα1 knockdown cells. (D) The interaction between endogenous PDHA and PDHK1 was determined in control and AMPKα1 knockdown cells upon A-769662 treatment. (E) Immunostaining of p-AMPK (T172) and p-PDHA (S293) in breast cancer tissues with different stages were shown. (F) Scatterplot of pAMPK expression versus pPDHA expression in breast cancer tissues was shown. (G) Kaplan-Meier plots showed high expression of pAMPK and low expression of pPDHA significantly predicted metastasis-free survival. Scale bars indicate 100 μm. See also Figure S4 and .

Article Snippet: Human breast cancer tissues , Chi Mei Medical Center from 1998 to 2004. IRB: 10210004 , N/A.

Techniques: Activation Assay, Phospho-proteomics, Knockdown, Control, Immunostaining, Expressing

Journal: Molecular Cell

Article Title: Phosphorylation of PDHA by AMPK Drives TCA Cycle to Promote Cancer Metastasis

doi: 10.1016/j.molcel.2020.09.018

Figure Lengend Snippet:

Article Snippet: Human breast cancer tissues , Chi Mei Medical Center from 1998 to 2004. IRB: 10210004 , N/A.

Techniques: Virus, Recombinant, Activity Assay, Colorimetric Assay, Stripping Membranes, Plasmid Preparation, Western Blot, Knock-Out, Software, Phospho-proteomics